Research Paper Volume 13, Issue 6 pp 7846—7871

Enhancing lifespan of budding yeast by pharmacological lowering of amino acid pools

Myr treatment maintains amino acid pools at a low level. (A) Amino acid levels in Myr-treated or untreated cells were measured over a 6 h time span following inoculation of stationary phase cells into fresh SDC medium. Metabolites were analyzed by using the heat-extraction, fluorescent-derivatization procedure. All data were statistically significant by AUC (95% CI: values for each pair of curves do not overlap; error bars are SEM). (B–D) Amino acid levels analyzed by using the GC-MS procedure. Amino acids are grouped into three panels according to their relative concentration. Significance determined by the Student’s t-test and error bars are SEM.

Figure 2. Myr treatment maintains amino acid pools at a low level. (A) Amino acid levels in Myr-treated or untreated cells were measured over a 6 h time span following inoculation of stationary phase cells into fresh SDC medium. Metabolites were analyzed by using the heat-extraction, fluorescent-derivatization procedure. All data were statistically significant by AUC (95% CI: values for each pair of curves do not overlap; error bars are SEM). (BD) Amino acid levels analyzed by using the GC-MS procedure. Amino acids are grouped into three panels according to their relative concentration. Significance determined by the Student’s t-test and error bars are SEM.