Research Paper Volume 13, Issue 6 pp 7846—7871

Enhancing lifespan of budding yeast by pharmacological lowering of amino acid pools

Mup1 biosynthesis and secretion appear unaffected by myriocin treatment. (A) JTY240 cells, with chromosomal copies of MUP1-GFP and VPH1-MARS, were gown to mid-log phase (A600nm = 0.4) and treated with Met (0.134 umol/L or 20 μg/ml) for 1 hour to deplete Mup1-GFP at the PM. Cells were washed with water, shifted to Met-deficient SC medium with or without Myr treatment for 1 hour and visualized by fluorescence microscopy. (B) JTY240 cells grown overnight in SC medium to stationary phase were washed with water and cultured (starting A600nm = 0.15) in fresh SC medium with or without Myr treatment.

Figure 4. Mup1 biosynthesis and secretion appear unaffected by myriocin treatment. (A) JTY240 cells, with chromosomal copies of MUP1-GFP and VPH1-MARS, were gown to mid-log phase (A600nm = 0.4) and treated with Met (0.134 umol/L or 20 μg/ml) for 1 hour to deplete Mup1-GFP at the PM. Cells were washed with water, shifted to Met-deficient SC medium with or without Myr treatment for 1 hour and visualized by fluorescence microscopy. (B) JTY240 cells grown overnight in SC medium to stationary phase were washed with water and cultured (starting A600nm = 0.15) in fresh SC medium with or without Myr treatment.