Research Paper Volume 13, Issue 6 pp 7846—7871

Enhancing lifespan of budding yeast by pharmacological lowering of amino acid pools

The Pib2 and Gtr1/2 pathways sense amino acid pools to control TORC1 activity and increase lifespan in Myr-treated cells. (A) Growth rate and cell density of indicated strains with or without Myr treatment. (B, C) The role of the Gtr1/2 pathway in lifespan was examined by measuring the viability of prototrophic WT BY4741, iml1∆ and npr2∆ cells, with and without Myr treatment, over time. (D) The role of the Pib2 pathway in lifespan was examined by measuring the viability of prototrophic BY4741 and pib2∆ cells, with and without Myr treatment, over time.

Figure 6. The Pib2 and Gtr1/2 pathways sense amino acid pools to control TORC1 activity and increase lifespan in Myr-treated cells. (A) Growth rate and cell density of indicated strains with or without Myr treatment. (B, C) The role of the Gtr1/2 pathway in lifespan was examined by measuring the viability of prototrophic WT BY4741, iml1∆ and npr2∆ cells, with and without Myr treatment, over time. (D) The role of the Pib2 pathway in lifespan was examined by measuring the viability of prototrophic BY4741 and pib2∆ cells, with and without Myr treatment, over time.