Research Paper Volume 13, Issue 7 pp 10703—10723

ASIC1 and ASIC3 mediate cellular senescence of human nucleus pulposus mesenchymal stem cells during intervertebral disc degeneration

Differential expression of ASICs in freshly isolated human IVD tissues with different degrees of intervertebral disc degeneration (IVDD). (A) Representative Western blotting analyses of ASICs 1-4 in IVD tissues from non-IVDD and IVDD groups from mild to severe cases. (B) Analyses of the relative expression of ASIC1 and ASIC3 determined for all patient IVD tissues (n=3 for each group) using densitometric analysis of Western blotting as per (A). Data are mean ± SD. * indicates P≤0.05 compared to non-IVDD group; # indicates P≤0.05 compared to mild group.

Figure 2. Differential expression of ASICs in freshly isolated human IVD tissues with different degrees of intervertebral disc degeneration (IVDD). (A) Representative Western blotting analyses of ASICs 1-4 in IVD tissues from non-IVDD and IVDD groups from mild to severe cases. (B) Analyses of the relative expression of ASIC1 and ASIC3 determined for all patient IVD tissues (n=3 for each group) using densitometric analysis of Western blotting as per (A). Data are mean ± SD. * indicates P≤0.05 compared to non-IVDD group; # indicates P≤0.05 compared to mild group.