Figure 2. Differential expression of ASICs in freshly isolated human IVD tissues with different degrees of intervertebral disc degeneration (IVDD). (A) Representative Western blotting analyses of ASICs 1-4 in IVD tissues from non-IVDD and IVDD groups from mild to severe cases. (B) Analyses of the relative expression of ASIC1 and ASIC3 determined for all patient IVD tissues (n=3 for each group) using densitometric analysis of Western blotting as per (A). Data are mean ± SD. * indicates P≤0.05 compared to non-IVDD group; # indicates P≤0.05 compared to mild group.