Research Paper Volume 13, Issue 7 pp 10703—10723
ASIC1 and ASIC3 mediate cellular senescence of human nucleus pulposus mesenchymal stem cells during intervertebral disc degeneration
Figure 3. Analyses of ASIC1 and ASIC3 expression in primary cultured human NP-MSCs. (A) Western blotting analyses of ASIC1 and ASIC3 expression when exposed to extracellular acid (pH6.6) with or without treatment of inhibitor Amiloride, PcTx1 or APETx2. (B) The relative expression of ASIC1 and ASIC3 using densitometric analysis of specific bands revealed by Western blotting as per (A). The samples were from case 4. Data are mean ± SD. With * indicating P≤0.05.