Research Paper Volume 13, Issue 7 pp 10703—10723

ASIC1 and ASIC3 mediate cellular senescence of human nucleus pulposus mesenchymal stem cells during intervertebral disc degeneration

The effect of acidic culture conditions on the proliferation of human NP-MSCs. The proliferation ability of human NP-MSCs derived from patients with Pfirrmann grade II (A), III (B) and IV (C) degeneration was determined using CCK-8 assays under control conditions or after exposure to extracellular acid (pH6.6) alone and in combination with addition of the inhibitors Amiloride, PcTx1 or APETx2. n=3 for each group; data are mean ± SD.

Figure 4. The effect of acidic culture conditions on the proliferation of human NP-MSCs. The proliferation ability of human NP-MSCs derived from patients with Pfirrmann grade II (A), III (B) and IV (C) degeneration was determined using CCK-8 assays under control conditions or after exposure to extracellular acid (pH6.6) alone and in combination with addition of the inhibitors Amiloride, PcTx1 or APETx2. n=3 for each group; data are mean ± SD.