Research Paper Volume 13, Issue 7 pp 10703—10723

ASIC1 and ASIC3 mediate cellular senescence of human nucleus pulposus mesenchymal stem cells during intervertebral disc degeneration

The production of secreted SASP cytokines by human NP-MSCs is increased by acidic culture and partially reversed by ASICs inhibition. ELISA assays measuring secreted IL-6 (A) and IL-8 (B) by human NP-MSCs after culture in control or acidic medium (pH 6.6) with or without treatment using the inhibitors Amiloride, PcTx1 or APETx2 as indicated. n=3; data are mean ±SD, * indicating P≤0.01 compared to control group at the same time points; # indicating P≤0.01 compared to pH 6.6 group at the same time points. The samples were from case 8.

Figure 7. The production of secreted SASP cytokines by human NP-MSCs is increased by acidic culture and partially reversed by ASICs inhibition. ELISA assays measuring secreted IL-6 (A) and IL-8 (B) by human NP-MSCs after culture in control or acidic medium (pH 6.6) with or without treatment using the inhibitors Amiloride, PcTx1 or APETx2 as indicated. n=3; data are mean ±SD, * indicating P≤0.01 compared to control group at the same time points; # indicating P≤0.01 compared to pH 6.6 group at the same time points. The samples were from case 8.