Figure 4. HucMSC-ex protect skin keratinocytes from oxidative stress. (A) The ROS production of HaCaT cells after 600μM H2O2 treatment was detected by the inverted fluorescence microscope. (B) Immunohistochemical staining of 8-OHDG expression level in HaCaT cells treated with 600μM H2O2. (C) Immunofluorescence detection of PCNA and Caspase3 expression level in HaCaT cells treated with 600μM H2O2. (D) qRT-PCR detection of the expression level of HaCaT cells inflammatory cytokines TNF-α (n = 3; *p < 0.05, **p < 0.01, ***p < 0.001).