Research Paper Volume 13, Issue 8 pp 11542—11563

HucMSC exosome-delivered 14-3-3ζ alleviates ultraviolet radiation-induced photodamage via SIRT1 pathway modulation

HucMSC-ex promote SIRT1 expression level under oxidative stress and activates autophagy to alleviate HaCaT cells damage. (A) Western blot analysis of SIRT1 expression level in HaCaT cells treated with different intensity of UVB. (B) Western blot analysis of SIRT1 expression level in HaCaT cells treated with different concentrations of H2O2. (C) Western blot detection of SIRT1 expression level in HaCaT cells treated with H2O2 at different times. (D) Western blot detection of SIRT1 expression level after H2O2 treatment of HaCaT cells at different times. (E) Immunofluorescence detection of SIRT1 expression level after H2O2 treatment of HaCaT cells. (F) qRT-PCR was used to detect the expression level of SIRT1 and Nrf2 mRNA of HaCaT cells after 24hours treatment with H2O2 (n = 3; *p G) Western blot detection of PCNA, Bax, LC3II, LC3I and SIRT1 protein expression level. (H) Immunofluorescence detection of hucMSC-ex effect on autophagy associated protein LC3B expression level.

Figure 5. HucMSC-ex promote SIRT1 expression level under oxidative stress and activates autophagy to alleviate HaCaT cells damage. (A) Western blot analysis of SIRT1 expression level in HaCaT cells treated with different intensity of UVB. (B) Western blot analysis of SIRT1 expression level in HaCaT cells treated with different concentrations of H2O2. (C) Western blot detection of SIRT1 expression level in HaCaT cells treated with H2O2 at different times. (D) Western blot detection of SIRT1 expression level after H2O2 treatment of HaCaT cells at different times. (E) Immunofluorescence detection of SIRT1 expression level after H2O2 treatment of HaCaT cells. (F) qRT-PCR was used to detect the expression level of SIRT1 and Nrf2 mRNA of HaCaT cells after 24hours treatment with H2O2 (n = 3; *p < 0.05, **p < 0.01, ***p < 0.001). (G) Western blot detection of PCNA, Bax, LC3II, LC3I and SIRT1 protein expression level. (H) Immunofluorescence detection of hucMSC-ex effect on autophagy associated protein LC3B expression level.