Research Paper Volume 13, Issue 8 pp 11542—11563

HucMSC exosome-delivered 14-3-3ζ alleviates ultraviolet radiation-induced photodamage via SIRT1 pathway modulation

HucMSC-ex delivered 14-3-3ζ protect HaCaT cells from oxidative stress in vitro. (A) Western blot analysis of 14-3-3ζ expression level in Ad-GFP-ex and Ad-14-3-3ζ-ex. (B) qRT-PCR quantification of SIRT1 mRNA expression level in HaCaT cells treated with Ad-14-3-3ζ-ex after 24h (n = 3; *p C) Immunofluorescence staining detection of SIRT1 expression level in HaCaT cells treated with Ad-14-3-3ζ-ex after 24hours. (D) Western blot analysis of SIRT1 expression level in HaCaT cells treated with Ad-14-3-3ζ-ex after 12hours and 24hours. (E) The DNA damage of HaCaT cells after treatment with Ad-14-3-3ζ-ex was detected by immunohistochemistry staining. (F) Western blot method for the detection of SIRT1 expression level after transfection of HaCaT cells with different titers of adenovirus. (G) Immunofluorescence staining detection of SIRT1 expression level after transfection of adenovirus into HaCaT cells.

Figure 6. HucMSC-ex delivered 14-3-3ζ protect HaCaT cells from oxidative stress in vitro. (A) Western blot analysis of 14-3-3ζ expression level in Ad-GFP-ex and Ad-14-3-3ζ-ex. (B) qRT-PCR quantification of SIRT1 mRNA expression level in HaCaT cells treated with Ad-14-3-3ζ-ex after 24h (n = 3; *p < 0.05, **p < 0.01, ***p < 0.001). (C) Immunofluorescence staining detection of SIRT1 expression level in HaCaT cells treated with Ad-14-3-3ζ-ex after 24hours. (D) Western blot analysis of SIRT1 expression level in HaCaT cells treated with Ad-14-3-3ζ-ex after 12hours and 24hours. (E) The DNA damage of HaCaT cells after treatment with Ad-14-3-3ζ-ex was detected by immunohistochemistry staining. (F) Western blot method for the detection of SIRT1 expression level after transfection of HaCaT cells with different titers of adenovirus. (G) Immunofluorescence staining detection of SIRT1 expression level after transfection of adenovirus into HaCaT cells.