Research Paper Volume 13, Issue 8 pp 11595—11609

Exosomes derived from mycobacterium tuberculosis-infected MSCs induce a pro-inflammatory response of macrophages

MSCs identification. (A) Detection of stem cell markers by flow cytometer. MSCs positively expressed CD29, CD44, CD73, CD90, CD271 and CD105, while negatively expressed CD31, CD45, and HLA-DR. (B) Morphology of MSCs under an inverted microscope. Scale bar: 20 μm. (C) Adipogenic differentiation of MSCs, as detected by oil red O staining. Scale bar: 20 μm. (D) Chondrogenesis differentiation of MSCs, as detected by Alcian Blue staining. Scale bar: 20 μm.

Figure 1. MSCs identification. (A) Detection of stem cell markers by flow cytometer. MSCs positively expressed CD29, CD44, CD73, CD90, CD271 and CD105, while negatively expressed CD31, CD45, and HLA-DR. (B) Morphology of MSCs under an inverted microscope. Scale bar: 20 μm. (C) Adipogenic differentiation of MSCs, as detected by oil red O staining. Scale bar: 20 μm. (D) Chondrogenesis differentiation of MSCs, as detected by Alcian Blue staining. Scale bar: 20 μm.