Research Paper Volume 13, Issue 8 pp 11646—11664

Two reactive behaviors of chondrocytes in an IL-1β-induced inflammatory environment revealed by the single-cell RNA sequencing

Cell distribution along pseudo-time trajectory and construction of the response-to-inflammation path. (A) A trajectory with three branches and one connecting points was constructed by Monocle 2. (B) The time-line of the pseudo-time trajectory; the darker blue indicates earlier pseudo-time. (C) The distribution of cluster2, 1, 7, and 0 along the pseudo-time trajectory. These cells compromised a complete evolutionary route without branches. (D) The distribution of cluster3, 5, 6, and 4 along the pseudo-time trajectory. These cells compromised a different evolutionary route from the former with differentiated branches. (E–G) The expression level of specific representative genes of terminal cluster 0 in cluster2, 1, 7, and 0 are highlighted along the response-to-inflammation path and real-time line. (F–H) The expression level of specific representative genes of terminal cluster 4 in cluster3, 5, 6, and 4 along the atypical response-to-inflammation path and real-time line.

Figure 3. Cell distribution along pseudo-time trajectory and construction of the response-to-inflammation path. (A) A trajectory with three branches and one connecting points was constructed by Monocle 2. (B) The time-line of the pseudo-time trajectory; the darker blue indicates earlier pseudo-time. (C) The distribution of cluster2, 1, 7, and 0 along the pseudo-time trajectory. These cells compromised a complete evolutionary route without branches. (D) The distribution of cluster3, 5, 6, and 4 along the pseudo-time trajectory. These cells compromised a different evolutionary route from the former with differentiated branches. (EG) The expression level of specific representative genes of terminal cluster 0 in cluster2, 1, 7, and 0 are highlighted along the response-to-inflammation path and real-time line. (FH) The expression level of specific representative genes of terminal cluster 4 in cluster3, 5, 6, and 4 along the atypical response-to-inflammation path and real-time line.