Research Paper Volume 13, Issue 8 pp 11665—11677

SPRY4 suppresses proliferation and induces apoptosis of colorectal cancer cells by repressing oncogene EZH2

Differential expression of SPRY4 and EZH2 between colorectal cancer cells and normal human colon cell. From four human CRC cell lines, SW620, SW480, LOVO and HCT116, and a normal human colon mucosal epithelial cell line NCM460, we detected the mRNA level and protein expression of SPRY4 using western blotting and RT-qPCR, respectively (A–C). The mRNA level and protein expression of EZH2 were detected using western blotting and RT-qPCR, respectively (D–F). **, ***p

Figure 1. Differential expression of SPRY4 and EZH2 between colorectal cancer cells and normal human colon cell. From four human CRC cell lines, SW620, SW480, LOVO and HCT116, and a normal human colon mucosal epithelial cell line NCM460, we detected the mRNA level and protein expression of SPRY4 using western blotting and RT-qPCR, respectively (AC). The mRNA level and protein expression of EZH2 were detected using western blotting and RT-qPCR, respectively (DF). **, ***p<0.01, 0.001 vs NCM460.