Figure 2. Effects of SPRY4 overexpression on CRC cell proliferation, migration and invasion. The overexpression plasmid of SPRY4 was constructed and transfected into SW480 cells. The mRNA level and protein expression of SPRY4 were detected using RT-qPCR and western blotting, respectively (A–C). Cell proliferation ability was determined using CCK-8 assay (D), cell colony formation (E), and EdU assay (F). Wound-healing and Transwell assays were conducted to measure cell migration and invasion, respectively (G–J). Protein expression of MMP2 and MMP9 was detected by western blotting (K–M). **, ***p<0.01, 0.001 vs Oe-NC.