Figure 5. The function of SPRY4 silence on proliferation, migration and invasion in SW480 cells. SW480 cells were transfected with si-SPRY4 plasmid-1/2 and the empty plasmid, then the protein expression of SPRY4 in different groups was measured using western blotting (A). After transfection, the protein expression of EZH2 was also measured using western blot (B). ***P<0.001 vs si-NC. SW480 cells were treated with GSK126 or co-treated with GSK126 and si-SPRY4. CCK-8 assay was conducted to determine cell proliferation ability (C). Wound-healing and Transwell assays were performed to determine cell migration and invasion abilities (D–G). The protein expression of MMP2 and MMP9 was measured using western blotting (H–J). ***p<0.001 vs Control; ##, ###p<0.01, 0.001 vs GSK126.