Figure 2. Auditory function of Piccolo-/- mice. (A) Gross morphology of Piccolo-/- and wild type (WT) mice at the age of one month (P30). Scale bar = 2cm. (B) Overall appearance of cochleae from Piccolo-/- and WT mice at P30. Scale bar = 15mm. (C) H&E staining of cochlear sections in Piccolo-/- and WT mice at P30. Scale bar = 20 μm. (D) Scanning electron microscopic images of cochlear hair bundles in Piccolo-/- and WT mice at P30. Scale bar = 5 μm. (E–G) ABR thresholds of Piccolo-/- (n = 3) and WT (n = 3) mice (at the age of one month, 4 months and 8 months, respectively) to click stimuli and to 4, 8, 16, and 32 kHz stimuli. Error bars = Means ± SD. (H) Representative ABR recording from control (n = 5) and Piccolo-/- (n = 3) mice at P30. The asterisk indicates a significant difference between WT and Piccolo-/- mice. *P < 0.05, **P < 0.01, by two-tailed Student’s t test. Error bars = Mean ± SD.