Research Paper Volume 13, Issue 6 pp 8026—8039

mTOR inhibition improves mitochondria function/biogenesis and delays cardiovascular aging in kidney transplant recipients with chronic graft dysfunction

(A) Boxplot representation of the ATP-dependent O2 consumption, measured in peripheral blood mononuclear cells (PBMC) from patients treated with calcineurin inhibitor (CNi) or CNi + mTOR inhibitor (CNi+mTORi) as absolute difference between that obtained in the absence and that in the presence of oligomycin (RR-OL) or normalized to basal respiration ((RR-OL)/RR). (B) Boxplot representation of the respiratory control ratio obtained by dividing the oxygen consumption rates measured in PBMCs from patients treated with CNi or CNi+mTORi under resting conditions by that in the presence of oligomycin (RR/OL). Statistical difference was assessed by unpaired student’s t-test.

Figure 3. (A) Boxplot representation of the ATP-dependent O2 consumption, measured in peripheral blood mononuclear cells (PBMC) from patients treated with calcineurin inhibitor (CNi) or CNi + mTOR inhibitor (CNi+mTORi) as absolute difference between that obtained in the absence and that in the presence of oligomycin (RR-OL) or normalized to basal respiration ((RR-OL)/RR). (B) Boxplot representation of the respiratory control ratio obtained by dividing the oxygen consumption rates measured in PBMCs from patients treated with CNi or CNi+mTORi under resting conditions by that in the presence of oligomycin (RR/OL). Statistical difference was assessed by unpaired student’s t-test.