Figure 4. (A) Percentage of peripheral blood mononuclear cells (PBMC) with polarized mitochondria from patients treated with calcineurin inhibitor (CNi) or CNi + mTOR inhibitor (CNi+mTORi). Mitochondrial polarization was detected by flow cytometry analysis of cells stained with JC-10. (B) Cytofluorimetric detection of reactive species in PBMCs from patients treated with CNi or CNi+mTORi, after staining with 2′,7′-dichlorofluorescein diacetate (DCFH-DA); AUF, arbitrary units of fluorescence. Statistical difference was assessed by unpaired student’s t-test.