Research Paper Volume 13, Issue 6 pp 8026—8039

mTOR inhibition improves mitochondria function/biogenesis and delays cardiovascular aging in kidney transplant recipients with chronic graft dysfunction

(A) Percentage of peripheral blood mononuclear cells (PBMC) with polarized mitochondria from patients treated with calcineurin inhibitor (CNi) or CNi + mTOR inhibitor (CNi+mTORi). Mitochondrial polarization was detected by flow cytometry analysis of cells stained with JC-10. (B) Cytofluorimetric detection of reactive species in PBMCs from patients treated with CNi or CNi+mTORi, after staining with 2′,7′-dichlorofluorescein diacetate (DCFH-DA); AUF, arbitrary units of fluorescence. Statistical difference was assessed by unpaired student’s t-test.

Figure 4. (A) Percentage of peripheral blood mononuclear cells (PBMC) with polarized mitochondria from patients treated with calcineurin inhibitor (CNi) or CNi + mTOR inhibitor (CNi+mTORi). Mitochondrial polarization was detected by flow cytometry analysis of cells stained with JC-10. (B) Cytofluorimetric detection of reactive species in PBMCs from patients treated with CNi or CNi+mTORi, after staining with 2′,7′-dichlorofluorescein diacetate (DCFH-DA); AUF, arbitrary units of fluorescence. Statistical difference was assessed by unpaired student’s t-test.