Research Paper Volume 13, Issue 8 pp 11727—11737

MiR-27a-3p suppresses cerebral ischemia-reperfusion injury by targeting FOXO1

MiR-27a-3p reverses OGD-induced G1 arrest in HT22 cells via mediation of FOXO1/p27 Kip1 axis. (A) Western blotting showing the protein expression of FOXO1 and p27 Kip1 in HT22 cells. (B, C) Relative expression levels of FOXO1 (B) and p27 Kip1 (C) normalized to GAPDH. (D) FACS analysis of HT22 cell cycling after PI staining. (E) Cell cycle profile showing distribution of cells in G0/G1, S or G2 phase. **P#P##P

Figure 5. MiR-27a-3p reverses OGD-induced G1 arrest in HT22 cells via mediation of FOXO1/p27 Kip1 axis. (A) Western blotting showing the protein expression of FOXO1 and p27 Kip1 in HT22 cells. (B, C) Relative expression levels of FOXO1 (B) and p27 Kip1 (C) normalized to GAPDH. (D) FACS analysis of HT22 cell cycling after PI staining. (E) Cell cycle profile showing distribution of cells in G0/G1, S or G2 phase. **P<0.01 vs. #P<0.05, ##P<0.01 vs. OGD.