Figure 8. Sestrin 1 overexpression alleviated the progression of PCOS in vivo. (A) Level of sestrin 1 in rat ovarian tissues were detected with RT-qPCR. (B) HE staining of ovarian tissues in each group. (C) The expression of sestrin 1 was detected with western blot. (D) Sestrin 1 expression was quantified. **P < 0.01 compared to the control group, ##P < 0.01 compared to the sestrin 1 siRNA2 group.