Research Paper Volume 13, Issue 10 pp 13571—13584

Long intergenic noncoding RNA 00665 promotes proliferation and inhibits apoptosis in colorectal cancer by regulating miR-126-5p

LINC00665 knockdown inhibits the proliferation of CRC cells. (A) The CellTiter 96® AQueous One Solution Cell Proliferation assay showed different growth curves of LOVO and RKO cells after LINC00665 knockdown (n = 6; *P B, C) Colony formation analysis showed that the rates of colony formation in the LINC00665-knockdown groups were reduced compared with the control (NC) (n = 6; *P D, E) Cell cycle-related protein (cyclin D1, CDK4, Rb, and p-Rb) expression levels in the control (NC) and LINC00665-knockdown groups were determined by Western blotting (n = 6; *P

Figure 2. LINC00665 knockdown inhibits the proliferation of CRC cells. (A) The CellTiter 96® AQueous One Solution Cell Proliferation assay showed different growth curves of LOVO and RKO cells after LINC00665 knockdown (n = 6; *P < 0.05 vs NC). (B, C) Colony formation analysis showed that the rates of colony formation in the LINC00665-knockdown groups were reduced compared with the control (NC) (n = 6; *P < 0.05 vs NC). (D, E) Cell cycle-related protein (cyclin D1, CDK4, Rb, and p-Rb) expression levels in the control (NC) and LINC00665-knockdown groups were determined by Western blotting (n = 6; *P < 0.05 vs NC).