Figure 4. LINC00665 is a direct target of miR-126-5p and is inversely related to miR-126-5p in CRC. (A) Predicted miR-126-5p binding sites in the LINC00665 sequence. (B) Subcellular fractionation and qPCR analysis confirmed that LINC00665 was largely enriched in the cytoplasm of cells. (C) qPCR showed LINC00665 expression in cells transfected with a miR-126-5p inhibitor or mimic (n = 6, *P < 0.05 vs NC). (D) Luciferase reporter assay revealed the binding relationship between LINC00665 and miR-126-5p (n = 6, *P < 0.05 vs NC). (E) miR-126-5p mRNA levels were plotted against LINC00665 expression in CRC specimens, demonstrating a significant negative correlation (two-tailed Pearson’s correlation, r = 0.4839; P < 0.01).