Research Paper Volume 13, Issue 10 pp 13571—13584

Long intergenic noncoding RNA 00665 promotes proliferation and inhibits apoptosis in colorectal cancer by regulating miR-126-5p

MiR-126-5p inhibits LINC00665 function. (A) CellTiter 96® AQueous One Solution Cell Proliferation assays of cells cotransfected with an NC mimic or miR-126-5p mimic and a control plasmid (pcDNA3.1) or LINC00665 expression plasmid (pcDNA3.1-LINC00665) (n = 6; *P P B, C) Cells cotransfected with the NC mimic or miR-126-5p mimic and LINC00665 expression plasmid were subjected to a colony formation assay (n = 6; *P P

Figure 5. MiR-126-5p inhibits LINC00665 function. (A) CellTiter 96® AQueous One Solution Cell Proliferation assays of cells cotransfected with an NC mimic or miR-126-5p mimic and a control plasmid (pcDNA3.1) or LINC00665 expression plasmid (pcDNA3.1-LINC00665) (n = 6; *P < 0.05 vs NC+pcDNA3.1; #P < 0.05 vs NC+pcDNA3.1-LINC00665). (B, C) Cells cotransfected with the NC mimic or miR-126-5p mimic and LINC00665 expression plasmid were subjected to a colony formation assay (n = 6; *P < 0.05 vs NC+pcDNA3.1; #P < 0.05 vs miR-126-5p +pcDNA3.1).