Figure 2. Chronic APAP exposure ameliorates the effect of age in liver alterations. (A, C) Representative images (10X and 20X) of H&E (A) and Oil Red O (C) staining performed on various sections of livers from 2 m/o, 14 m/o and 14 m/o + APAP mice. (B) Steatosis NAS score, inflammation NAS score, and total NAS score. (D) Plasma ALT, AST and LDH levels from 2 m/o, 14 m/o and 14 m/o + APAP mice. Values are expressed in international units per liter (U/L). Data are represented as the mean ± S.E.M. (n = 6-18 mice per group). Statistical analysis was performed by one-way ANOVA or Brown-Forsythe and Welch ANOVA test followed by their respective post-hoc test. * P < 0.05, ** P < 0.01 and *** P < 0.001 vs. 2 m/o; #P < 0.05 and ##P < 0.01 vs. 14 m/o.