Figure 5. Double immunofluorescence staining of IL-6. The double immunofluorescence staining shows IL-6 is co-localized with IB4 (a marker for C-type neurons) (A), CGRP (a marker for small nociceptive peptidergic neurons) (B) and NF-200 (a marker for A-type neurons) (C) but not with GFAP (a marker for satellite glial cells) (D). With calculation, the percentage of IL-6 in IB4, CGRP, NF200 neuron was 72.3±8.1%, 81.9±8.9%, 69.1±9.2% respectively (E). The red refers to IL-6, the green refers to IB4, CGRP, NF-200 or GFAP, and the yellow refers to the colocalization.