Research Paper Volume 13, Issue 8 pp 11907—11918

GEO data mining and TCGA analysis reveal altered branched chain amino acid metabolism in pancreatic cancer patients

Examination of genes related to BCAA and fatty acid metabolism in PDAC tissue. A–F: Data mining in GSE62165 dataset. (A) The expression level of SLC carriers (SLC7A5, SLC1A5, SLC3A2, SLC43A1 and SLC43A2) between PDAC and normal tissue. (B) GSEA analysis revealed enriched MYC and NOTCH signaling pathway in PDAC tissue. (C) The expression level of MYC and NOTCH (NOTCH1-4) between PDAC and normal tissue. (D) BCAA catabolism related genes BCAT1, BCAT2, BCKDHA, BCKDHB, BCKDK, ACADS and ACADSB were found significantly down-regulated in PDAC group. (E) The expression of lipogenesis related enzymes including FAS, ACACA and ACLY were significantly up-regulated in PDAC tissue. (F) CPT1A, the rate-limiting enzyme for fatty acid oxidation was notably upregulated in PDAC tissue. G–J: Data mining in GSE102238 dataset. (G) The expression level of SLC carriers (SLC7A5, SLC1A5, SLC3A2, SLC43A1 and SLC43A2) between PDAC and adjacent normal tissue. (H) NOTCH3 and NOTCH4 were upregulated in PDAC tissue while MYC was down-regulated. (I) Gene expression of BCAT1, BCAT2, BCKDHA, BCKDHB and ACADSB were decreased in PDAC tissue. (J) FAS and ACACA were increased in PDAC tissue.

Figure 2. Examination of genes related to BCAA and fatty acid metabolism in PDAC tissue. AF: Data mining in GSE62165 dataset. (A) The expression level of SLC carriers (SLC7A5, SLC1A5, SLC3A2, SLC43A1 and SLC43A2) between PDAC and normal tissue. (B) GSEA analysis revealed enriched MYC and NOTCH signaling pathway in PDAC tissue. (C) The expression level of MYC and NOTCH (NOTCH1-4) between PDAC and normal tissue. (D) BCAA catabolism related genes BCAT1, BCAT2, BCKDHA, BCKDHB, BCKDK, ACADS and ACADSB were found significantly down-regulated in PDAC group. (E) The expression of lipogenesis related enzymes including FAS, ACACA and ACLY were significantly up-regulated in PDAC tissue. (F) CPT1A, the rate-limiting enzyme for fatty acid oxidation was notably upregulated in PDAC tissue. GJ: Data mining in GSE102238 dataset. (G) The expression level of SLC carriers (SLC7A5, SLC1A5, SLC3A2, SLC43A1 and SLC43A2) between PDAC and adjacent normal tissue. (H) NOTCH3 and NOTCH4 were upregulated in PDAC tissue while MYC was down-regulated. (I) Gene expression of BCAT1, BCAT2, BCKDHA, BCKDHB and ACADSB were decreased in PDAC tissue. (J) FAS and ACACA were increased in PDAC tissue.