Figure 5. miR-1246 facilitates the cell survival and impedes differentiation of LSCs by targeting LRIG1. LSCs were transfected with mimic-NC, miR-1246 mimic, miR-1246 mimic + LRIG1-NC, miR-1246 mimic + LRIG1, si-NC and si-LRIG1. (A, B) Expression of miR-1246 and LRIG1 in LSCs detected by RT-qPCR after transfection. (C) LSCs viability detected by MTS assay. (D) Colony formation of LSCs evaluated by colony formation assay. (E) LSCs apoptosis identified by flow cytometry. (F) Differentiation of LSCs determined by differentiation assay. * p < 0.05 vs. LSCs transfected with mimic-NC, miR-1246-mimic + LRIG1-NC or si-NC. Data in the figures are all measurement data, expressed as mean ± standard deviation. Data among multiple groups were analyzed by one-way ANOVA. The experiments were repeated in triplicate.