Research Paper Volume 13, Issue 10 pp 13644—13662

microRNA-1246-containing extracellular vesicles from acute myeloid leukemia cells promote the survival of leukemia stem cells via the LRIG1-meditated STAT3 pathway

AML cell-derived EVs deliver miR-1246 inhibitor to restrain the growth of transplantation tumors of LSCs. Mice were subcutaneously injected with normal saline, EVs-inhibitor-NC and EVs-miR-1246 inhibitor. (A, B) Tumor formation in mice observed and recorded in real time. (C) Expression of miR-1246 and LRIG1 in tumor tissues of mice tested by RT-qPCR. (D) Expression of LRIG1 and STAT3-related proteins in tumor tissues of mice detected by western blot analysis. * p vs. mice injected with normal saline. #p vs. mice injected with EVs-inhibitor-NC. n = 6. Data in the figures are all measurement data, expressed as mean ± standard deviation. Data among multiple groups were analyzed by one-way ANOVA. Data among multiple groups at different time points were measured by repeated measurement ANOVA.

Figure 7. AML cell-derived EVs deliver miR-1246 inhibitor to restrain the growth of transplantation tumors of LSCs. Mice were subcutaneously injected with normal saline, EVs-inhibitor-NC and EVs-miR-1246 inhibitor. (A, B) Tumor formation in mice observed and recorded in real time. (C) Expression of miR-1246 and LRIG1 in tumor tissues of mice tested by RT-qPCR. (D) Expression of LRIG1 and STAT3-related proteins in tumor tissues of mice detected by western blot analysis. * p < 0.05 vs. mice injected with normal saline. #p < 0.05 vs. mice injected with EVs-inhibitor-NC. n = 6. Data in the figures are all measurement data, expressed as mean ± standard deviation. Data among multiple groups were analyzed by one-way ANOVA. Data among multiple groups at different time points were measured by repeated measurement ANOVA.