Research Paper Volume 13, Issue 7 pp 10749—10769

Dual activation of Hedgehog and Wnt/β-catenin signaling pathway caused by downregulation of SUFU targeted by miRNA-150 in human gastric cancer

Negative correlation between miRNA-150 and SUFU in GC. MiRNA-150 is highly expressed in GC. The expression of SUFU is downregulated in GC and negatively correlated with the expression of miRNA-150. (A, B) The expression levels of miRNA-150 in 50 paired GC tissues are shown. (C) The expression levels of miRNA-150 in GC cell lines, namely BGC-823,AGS,SGC7901,N87 and MKN28 cells and normal epithelial cells (HFE-145, 293T, GES-1) were determined. (D, E) The expression levels of SUFU in GC cell lines were determined using western blotting and qPCR. (F) The expression levels of SUFU in 34 paired GC samples are shown. (G) A correlation analysis of miRNA-150 and SUFU mRNA levels was performed in 34 paired GC tissues.

Figure 2. Negative correlation between miRNA-150 and SUFU in GC. MiRNA-150 is highly expressed in GC. The expression of SUFU is downregulated in GC and negatively correlated with the expression of miRNA-150. (A, B) The expression levels of miRNA-150 in 50 paired GC tissues are shown. (C) The expression levels of miRNA-150 in GC cell lines, namely BGC-823,AGS,SGC7901,N87 and MKN28 cells and normal epithelial cells (HFE-145, 293T, GES-1) were determined. (D, E) The expression levels of SUFU in GC cell lines were determined using western blotting and qPCR. (F) The expression levels of SUFU in 34 paired GC samples are shown. (G) A correlation analysis of miRNA-150 and SUFU mRNA levels was performed in 34 paired GC tissues.