Research Paper Volume 13, Issue 7 pp 10749—10769

Dual activation of Hedgehog and Wnt/β-catenin signaling pathway caused by downregulation of SUFU targeted by miRNA-150 in human gastric cancer

SUFU mediates the carcinogenic action of miRNA-150. (A) MiRNA-150 inhibition decreased BGC-823 cell proliferation, and SUFU siRNAs rescued this inhibition. Cell proliferation was measured 48 h after transfection with miRNA-150 inhibitors or co-transfection with 150-inh and SUFU siRNAs. (B, C) MiRNA-150 inhibition suppressed the migratory ability of BGC-823 cells, whereas SUFU siRNAs restored this ability. (B) A wound healing assay was performed 48 h after transfection. (C) A Transwell assay was conducted 48 h after transfection. Five randomly chosen fields in each well were counted. (D) The colony-forming ability of cells was impaired by miRNA-150 inhibitors, while SUFU siRNAs restored this ability. A colony formation assay was performed 48 h after transfection with miRNA-150 inhibitors or co-transfection with 150-inh and SUFU siRNAs. Each experiment was repeated three times. *p

Figure 7. SUFU mediates the carcinogenic action of miRNA-150. (A) MiRNA-150 inhibition decreased BGC-823 cell proliferation, and SUFU siRNAs rescued this inhibition. Cell proliferation was measured 48 h after transfection with miRNA-150 inhibitors or co-transfection with 150-inh and SUFU siRNAs. (B, C) MiRNA-150 inhibition suppressed the migratory ability of BGC-823 cells, whereas SUFU siRNAs restored this ability. (B) A wound healing assay was performed 48 h after transfection. (C) A Transwell assay was conducted 48 h after transfection. Five randomly chosen fields in each well were counted. (D) The colony-forming ability of cells was impaired by miRNA-150 inhibitors, while SUFU siRNAs restored this ability. A colony formation assay was performed 48 h after transfection with miRNA-150 inhibitors or co-transfection with 150-inh and SUFU siRNAs. Each experiment was repeated three times. *p<.05.