Figure 3. Agonism of GPR30 using its specific agonist G1 suppressed oscillatory shear stress (OSS)-induced oxidative stress in HAECs. HAECs were exposed with OSS (5 dyn/cm2) in the presence or absence of 5, 10 μM G1 for 24 h. (A). Levels of intracellular reactive oxygen species (ROS) were determined by DCFH-DA staining; (B). Levels of intracellular 4-hydroxynonenal (4-HNE) were determined by immunostaining (*, #, $, P<0.01 vs. previous column group, N=5).