Figure 5. Agonism of GPR30 using its specific agonist G1 suppressed oscillatory shear stress (OSS)-induced expression of VCAM-1 and E-selectin in human aortic endothelial cells (HAECs). HAECs were exposed with OSS (5 dyn/cm2) in the presence or absence of 5, 10 μM G1 for 24 h. (A). Expression of VCAM-1 and E-selectin at the mRNA level; (B). Expression of VCAM-1 and E-selectin at the protein level was determined by ELISA (*, #, $, P<0.01 vs. previous column group, N=5).