Figure 7. Agonism of GPR30 using its specific agonist G1 rescued oscillatory shear stress (OSS)-induced reduction of KLF2. HAECs were exposed with OSS (5 dyn/cm2) in the presence or absence of 5, 10 μM G1 for 24 h. (A). Expression of KLF2 at the mRNA level was determined by real time PCR analysis; (B). Expression of KLF2 at the protein level was determined by western blot analysis (*, #, $, P<0.01 vs. previous column group, N=5).