Research Paper Volume 13, Issue 9 pp 12466—12478

Supplementary research on K150del variant of activated protein C

Establishment and characterization of HEK 293T cell lines. (A) Wild or mutant protein C (PC) zymogen and green fluorescent protein (GFP) were co-expressed in HEK 293T cells using lentiviral vectors. GFP-positive clones were observed under a fluorescence microscope to evaluate the efficacy of two transfections. (B) Differentially expressed genes (DEGs) were identified between APC-WT and APC-DEL groups. Each dot represents an individual gene. The horizontal axis indicates the expression (log10) of the genes in the APC-WT group, whereas the vertical axis refers to gene expression (log10) in the APC-DEL group. Red dots represent upregulated genes, blue dots represent downregulated genes, and black dots represent unaltered genes. The significance level for the difference in the expression of each gene between the two groups was set at adjusted P

Figure 1. Establishment and characterization of HEK 293T cell lines. (A) Wild or mutant protein C (PC) zymogen and green fluorescent protein (GFP) were co-expressed in HEK 293T cells using lentiviral vectors. GFP-positive clones were observed under a fluorescence microscope to evaluate the efficacy of two transfections. (B) Differentially expressed genes (DEGs) were identified between APC-WT and APC-DEL groups. Each dot represents an individual gene. The horizontal axis indicates the expression (log10) of the genes in the APC-WT group, whereas the vertical axis refers to gene expression (log10) in the APC-DEL group. Red dots represent upregulated genes, blue dots represent downregulated genes, and black dots represent unaltered genes. The significance level for the difference in the expression of each gene between the two groups was set at adjusted P < 0.01 and log2FC ≥ 1 or ≤ −1 (FC: gene expression changes).