Research Paper Volume 13, Issue 8 pp 12031—12045

Low magnitude vibration alleviates age-related bone loss by inhibiting cell senescence of osteogenic cells in naturally senescent rats

Senescence identification of osteocytes in aged and young rats. (A) 53BP1 immunofluorescence staining of aged rat and young rat osteocytes; (B) H3K9Me3 immunofluorescence staining of aged rat and young rat osteocytes; (C) Senescence marker protein assay and protein relative expression statistics of aged rat and young rat osteocytes (x¯±s,n=3); (D) β-Galactosidase staining of aged rat and young rat osteocytes and statistical plots of the percentage of positive β-galactosidase staining cells (x¯±s,n=3); *P #P ##P

Figure 4. Senescence identification of osteocytes in aged and young rats. (A) 53BP1 immunofluorescence staining of aged rat and young rat osteocytes; (B) H3K9Me3 immunofluorescence staining of aged rat and young rat osteocytes; (C) Senescence marker protein assay and protein relative expression statistics of aged rat and young rat osteocytes (x¯±s,n=3); (D) β-Galactosidase staining of aged rat and young rat osteocytes and statistical plots of the percentage of positive β-galactosidase staining cells (x¯±s,n=3); *P < 0.05 vs. young female rats, **P < 0.01 vs. young female rats, #P < 0.05 vs. young male rats, ##P < 0.01 vs. young male rats.