Research Paper Volume 13, Issue 8 pp 12046—12057

Hsa-miR-107 regulates chemosensitivity and inhibits tumor growth in hepatocellular carcinoma cells

MicroRNA-107 (miR-107) decreased cell survival and induced cell death in hepatocellular carcinoma (HCC) cells. (A) Regulation of miR-107 expression by transfection with mimic and inhibitor in HA22T and HDACi-R (histone deacetylase inhibitor-resistant) cells detected by quantitative RT-PCR. (B) MiR-107 decreased cell viability in HA22T and HDACi-R cells, as assessed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay. (C) MiR-107 promoted apoptosis-related proteins such as cleaved caspase-3 and decreased expression of pro-survival-related proteins. (D) Quantification of Figure 2C. Expression of fold change of cleavage caspase-3, Bcl-2, and p-Akt after normalization using actin. *P ***P #P ###P

Figure 2. MicroRNA-107 (miR-107) decreased cell survival and induced cell death in hepatocellular carcinoma (HCC) cells. (A) Regulation of miR-107 expression by transfection with mimic and inhibitor in HA22T and HDACi-R (histone deacetylase inhibitor-resistant) cells detected by quantitative RT-PCR. (B) MiR-107 decreased cell viability in HA22T and HDACi-R cells, as assessed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay. (C) MiR-107 promoted apoptosis-related proteins such as cleaved caspase-3 and decreased expression of pro-survival-related proteins. (D) Quantification of Figure 2C. Expression of fold change of cleavage caspase-3, Bcl-2, and p-Akt after normalization using actin. *P < 0.05, ***P < 0.001 vs. HA22T control. #P < 0.05, ###P < 0.001 vs. HDACi-R control.