Research Paper Volume 13, Issue 8 pp 12067—12085

Expression profile, molecular functions, and prognostic significance of miRNAs in primary colorectal cancer stem cells

The GO and KEGG pathways of pCRCSC-related miRNAs. (A) The top 15 biological processes, (B) cell components, (C) molecular functions, and (D) the KEGG pathways of target genes based on the intersection of predicted downstream target genes and genes that negatively correlated with miRNA expression in the TCGA–COREAD dataset. (E) Nine potential target genes of pCRCSC-related miRNAs were validated by RT-qPCR in the primary CSCs derived from colon cancer and the corresponding differentiated cells. (F) Nine potential target genes of pCRCSC-related miRNAs were validated by RT-qPCR in the primary CSCs derived from rectal cancer and the corresponding differentiated cells. GAPDH was selected as the internal control. This experiment was repeated thrice. Bars represent mean ± standard deviation (SD) (n = 3, *p **p ***p

Figure 4. The GO and KEGG pathways of pCRCSC-related miRNAs. (A) The top 15 biological processes, (B) cell components, (C) molecular functions, and (D) the KEGG pathways of target genes based on the intersection of predicted downstream target genes and genes that negatively correlated with miRNA expression in the TCGA–COREAD dataset. (E) Nine potential target genes of pCRCSC-related miRNAs were validated by RT-qPCR in the primary CSCs derived from colon cancer and the corresponding differentiated cells. (F) Nine potential target genes of pCRCSC-related miRNAs were validated by RT-qPCR in the primary CSCs derived from rectal cancer and the corresponding differentiated cells. GAPDH was selected as the internal control. This experiment was repeated thrice. Bars represent mean ± standard deviation (SD) (n = 3, *p < 0.05, **p < 0.01, ***p < 0.001).