Research Paper Volume 13, Issue 8 pp 12113—12128

LncRNA PlncRNA-1 accelerates the progression of prostate cancer by regulating PTEN/Akt axis

Effect of PlncRNA-1 knockdown on the proliferation, migration, and invasion of PCa cells. (A) qPCR analysis for the transfection efficiency of PlncRNA-1-siRNA in 22Rv1 and DU145 PCa cells. (B–C) CCK-8 assay for the proliferation of 22Rv1 and DU145 PCa cells after PlncRNA-1 silencing. (D–G) Wound healing assays for the migration of 22Rv1 and DU145 PCa cells after PlncRNA-1 silencing (Scale bar, 200 μm). (H) Transwell migration and invasion assays for the migration and invasion of 22Rv1 and DU145 PCa cells after PlncRNA-1 silencing (Scale bar, 100 μm). (I–J) Quantitative analysis transwell migration (I) and invasion (J) assays of 22Rv1 and DU145 PCa cells after PlncRNA-1 silencing.

Figure 2. Effect of PlncRNA-1 knockdown on the proliferation, migration, and invasion of PCa cells. (A) qPCR analysis for the transfection efficiency of PlncRNA-1-siRNA in 22Rv1 and DU145 PCa cells. (BC) CCK-8 assay for the proliferation of 22Rv1 and DU145 PCa cells after PlncRNA-1 silencing. (DG) Wound healing assays for the migration of 22Rv1 and DU145 PCa cells after PlncRNA-1 silencing (Scale bar, 200 μm). (H) Transwell migration and invasion assays for the migration and invasion of 22Rv1 and DU145 PCa cells after PlncRNA-1 silencing (Scale bar, 100 μm). (IJ) Quantitative analysis transwell migration (I) and invasion (J) assays of 22Rv1 and DU145 PCa cells after PlncRNA-1 silencing.