Figure 5. SUMO1P3 knockdown inhibited HCC cell migration and invasion in vitro. MHCC97H and HepG2 cells were untransfected (control) or transfected with siSUMO1P3 or siNC. Transwell assays were performed to evaluate the migration (A) and invasion (C) of MHCC97H and HepG2 cells. 40 × magnification in (C). The numbers of migrated (B) and invaded (D) cells were calculated. (E) The expression of E-cadherin, vimentin, MMP-2, and MMP-9 was analyzed via Western blot. β-actin was used as endogenous control. All data are represented as the mean ± SD of three replicates. *P < 0.05 vs. Ctrl or siNC group.