Figure 5. U-slide chemotaxis experiments to observe neutrophil viability, migration, and clustering ability that was affected by advanced glycation end products (AGEs). Scale bar as 1 mm. (A) Measurement of cell migration and aggregation. The blue and red squares represent middle-range and near-range areas, respectively. The blue circle represents neutrophil aggregation (clustering). (B, G) Neutrophil clustering ability that was affected by AGEs stimulation and suspension. (C, E) Neutrophil migration ability that was affected by AGEs stimulation at different time points. (D, F) Neutrophil migration ability that was affected by AGEs resuspension. *: P<0.05, **: P<0.01, ***: P<0.001.