Figure 1. RANKL-Induced osteoclast formation in vitro was down-regulated by RO4929097. (A) RO4929097cytotoxicity. (B) The results showed that the IC50 of RO4929097 at different time points in the BMMs was 4.44 μM, 3.66 μM, and 2.93 μM, respectively. (C) The chemical structural formula of RO4929097 (provided by Selleck). (D) BMMs were simulated with different concentrations of RO4929097 combined with M-CSF (33.3 ng/mL) and RANKL (100 ng/mL) for five days. Cells were fixed in 4% paraformaldehyde and stained for TRAP. (E and F) The area and number of TRAP-positive cells. The data were presented as the mean ± SD (*p < 0.05, **p < 0.01, ***p < 0.001).