Figure 2. Podosome actin belt formation and OC-mediated bone resorption activity was inhibited by RO4929097. (A) OCL were seeded onto Osteo Assay Stripwell Plates with RANKL (100 ng/mL), M-CSF (33.3 ng/mL), and RO4929097 (0,100, 200, and 400 nM) for four days (B) The resorption area in Osteo Assay Stripwell Plates. (C) BMMs were cultured in 48-well plates with 0, 100, 200, and 400 nM RO4929097 for five days. The cells were fixed and stained for immunofluorescence. (D) Area of OCs with an F-actin belt. The data were presented as the mean ± SD.