Research Paper Volume 13, Issue 9 pp 12537—12551

Silencing long noncoding RNA NEAT1 alleviates acute liver failure via the EZH2-mediated microRNA-139/PUMA axis

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Figure 2. LncRNA NEAT1 was upregulated in ALF. (A) RT-qPCR was used to analyze the lncRNA NEAT1 levels in human serum samples. Healthy control (HC): n = 11. ALF: n = 35. (B) The expression levels of lncRNA NEAT1 was determined by RT-qPCR in liver tissues from control mice and D-GalN/LPS-induced ALF mice. (C) Results of RT-qPCR for determining the lncRNA NEAT1 level in human PBMCs with or without 10 ng/mL LPS treatment. The expression level of lncRNA NEAT1 was normalized to the control groups. Data were summarized as mean ± S.D. from at least 3 independent biological replicates and * indicates p < 0.05. Data were compared using the unpaired student’s t-test.