Figure 3. Silencing NEAT1 alleviated the occurrence of ALF. (A) Results of RT-qPCR for determining the knockdown efficiency of sh-NEAT1. (B) H&E staining to study histopathological changes in the liver after silencing NEAT1. (C) Biochemical markers ALT and AST activities in ALF mouse serum. (D) The mRNA expression levels of TNF-α, IL-6, and IL-1β were determined by RT-qPCR and normalized to sh-NC group. (E) ELISA assay to determine the cytokine level in ALF mice. Data were summarized as mean ± S.D. from at least 3 independent biological replicates. One-way ANOVA was applied to compare data among multiple groups. * indicates p < 0.05. N = 12 mice for each group.