Research Paper Volume 13, Issue 9 pp 12537—12551

Silencing long noncoding RNA NEAT1 alleviates acute liver failure via the EZH2-mediated microRNA-139/PUMA axis

LncRNA NEAT1 suppressed the expression of miR-139 via EZH2 and induced cytokine production. (A) RT-qPCR to determine the expression levels of miR-139 and EZH2 in serum samples of patients with ALF, ALF murine liver tissues, and LPS-induced PBMCs. (B) Western blot analysis results to detect the protein levels of EZH2 in ALF murine liver tissues, and LPS-induced PBMCs. (C) Expression of miR-139 upon knockdown and overexpression of lncRNA NEAT in LPS-treated PBMCs. (D) Interaction between EZH2 and NEAT1 as detected by RIP assay.

Figure 4A-D. LncRNA NEAT1 suppressed the expression of miR-139 via EZH2 and induced cytokine production. (A) RT-qPCR to determine the expression levels of miR-139 and EZH2 in serum samples of patients with ALF, ALF murine liver tissues, and LPS-induced PBMCs. (B) Western blot analysis results to detect the protein levels of EZH2 in ALF murine liver tissues, and LPS-induced PBMCs. (C) Expression of miR-139 upon knockdown and overexpression of lncRNA NEAT in LPS-treated PBMCs. (D) Interaction between EZH2 and NEAT1 as detected by RIP assay.