Figure 4E-I. LncRNA NEAT1 suppressed the expression of miR-139 via EZH2 and induced cytokine production. (E) ChIP analysis to determine the enrichment level of H3K27me3 on the promoter region of miR-139 upon knockdown and overexpression of lncRNA NEAT. (F) RT-qPCR to determine the expression levels of miR-139 and EZH2 in LPS-treated PBMCs in the presence of sh-NEAT1 with or without oe-EZH2. (G) Western blot analysis to determine the protein levels of EZH2. (H) mRNA levels of pro-inflammatory cytokines as detected by RT-qPCR. (I) ELISA analysis of cytokines levels. Data were summarized as mean ± S.D. from at least 3 independent biological replicates. (A, B) Data were compared using unpaired student’s t-test. (C–I) One-way ANOVA with Tukey’s post hoc test was applied to compare data among multiple groups. * indicates p < 0.05.