Figure 5A-D. miR-139 suppressed production of pro-inflammatory cytokines by targeting PUMA. (A) Prediction of binding of miR-139 to PUMA-3’UTR by TargetScan database (http://www.targetscan.org/vert_71/). (B) RT-qPCR to determine the expression levels of PUMA in serum samples of patients with ALF, ALF murine liver tissues, and LPS-induced PBMCs. (C) Results of Western blot analysis to determine the protein levels of PUMA in ALF murine liver tissues, and LPS-induced PBMCs. (D) Dual-luciferase reporter gene assays to analyze the target relationship between miR-139 and PUMA.