Research Paper Volume 13, Issue 9 pp 12537—12551

Silencing long noncoding RNA NEAT1 alleviates acute liver failure via the EZH2-mediated microRNA-139/PUMA axis

miR-139 suppressed production of pro-inflammatory cytokines by targeting PUMA. (E, F) PUMA mRNA (E) and protein (F) level in the transfected cells. (G) mRNA level of pro-inflammatory cytokines as determined by RT-qPCR. (H) ELISA analysis of cytokines levels. Data were summarized as mean ± S.D. from at least 3 independent biological replicates. (B–D) Data were compared using unpaired student’s t-test. (E–H) One-way ANOVA with Tukey’s post hoc test was applied to compare data among multiple groups. * indicates p

Figure 5E-H. miR-139 suppressed production of pro-inflammatory cytokines by targeting PUMA. (E, F) PUMA mRNA (E) and protein (F) level in the transfected cells. (G) mRNA level of pro-inflammatory cytokines as determined by RT-qPCR. (H) ELISA analysis of cytokines levels. Data were summarized as mean ± S.D. from at least 3 independent biological replicates. (BD) Data were compared using unpaired student’s t-test. (EH) One-way ANOVA with Tukey’s post hoc test was applied to compare data among multiple groups. * indicates p < 0.05.