Figure 6. Silencing of lncRNA NEAT1 attenuates the development of ALF through PUMA. (A) RT-qPCR analysis of lncRNA NEAT1, EZH2, miR-139, and PUMA levels in ALF mice. (B) Histological analysis of ALF mouse liver tissues. (C) Biochemical analysis of ALT and AST activity in ALF mouse serum samples. (D) Pro-inflammatory cytokines TNF-α, IL-6, and IL-1β mRNA level as detected by RT-qPCR. (E) ELISA assay performed to detect the TNF-α, IL-6 and IL-1β levels. Data were summarized as mean ± S.D. from at least 3 independent biological replicates. One-way ANOVA with Tukey’s post hoc test was applied to compare data among multiple groups. * indicates p < 0.05. n = 12 mice for each group.