Research Paper Volume 13, Issue 8 pp 12160—12178

Balasubramide derivative 3C attenuates atherosclerosis in apolipoprotein E-deficient mice: role of AMPK-STAT1-STING signaling pathway

3C protects ApoE-/- mice from high-fat diet-induced atherosclerotic plaque formation. (A) Chemical structure of compound 3C. (B) Representative images in situ of aortic arch and its branches with white plaques. (C) Representative images of the Enface staining with oil red-O of the whole aorta and quantification of Oil Red O–stained Enface aorta of plaque area for ApoE-/- mice treated with vehicle or 3C. (D) Representative images of plaques in the aortic root after 10 weeks of HFD was determined by staining sections with Oil red O. (E) Quantification of Oil Red O–stained aortic root for the lipid disposition area (n=5 per group). (F) Representative images of plaques in the aortic root staining sections with H&E staining. (G) Quantification of H&E-stained aortic root for the plaque lesion area and mean necrotic area (n=5 per group). All data were assessed using Student’s t-test and are present as mean±SEM. *P **P

Figure 1. 3C protects ApoE-/- mice from high-fat diet-induced atherosclerotic plaque formation. (A) Chemical structure of compound 3C. (B) Representative images in situ of aortic arch and its branches with white plaques. (C) Representative images of the Enface staining with oil red-O of the whole aorta and quantification of Oil Red O–stained Enface aorta of plaque area for ApoE-/- mice treated with vehicle or 3C. (D) Representative images of plaques in the aortic root after 10 weeks of HFD was determined by staining sections with Oil red O. (E) Quantification of Oil Red O–stained aortic root for the lipid disposition area (n=5 per group). (F) Representative images of plaques in the aortic root staining sections with H&E staining. (G) Quantification of H&E-stained aortic root for the plaque lesion area and mean necrotic area (n=5 per group). All data were assessed using Student’s t-test and are present as mean±SEM. *P <0.05; **P<0.01.